Fig. 9.

MAP2-targeting signal drives tau expression into the dendrites of differentiated P19 cells. Confocal image of a P19 cell line transfected GFP-tau–cod-MAP2-targeting signal. a, Localization of GFP-tau protein in the dendrites. b, P19 cell stained with MAP2 antibodies to visualize the dendrites.c, Localization by in situ hybridization of GFP-tau mRNA with a GFP probe detected with anti-dig HRP/Cy5.d, Merged image of a, b, and c showing the colocalization of GFP-tau protein and mRNA in the dendrites. e, Phase view. Note that the axon seen in e is not stained in a–d because tau is driven to the dendrites. Scale bar, 10 μm. Large filled arrowheads denote an axon, large open arrowheads denote dendrites, and small filled arrowheads denote a neuronal cell body.