Fig. 8.

After the coinfusion of BDNF and BrdU into the lateral ventricle of the adult rat brain, newly generated cells in the SVZ and within the parenchyma express a neuronal phenotype.A–K, To analyze the phenotype of the newly generated cells, 20 μm coronal sections were immunostained with anti-BrdU (red) and the neuronal antibody TuJ1 (green) (A–G) or anti-MAP-2 (green) (H–K) and then visualized with either conventional (A,F, G, J,K) or confocal (B–E,H, I) microscopy.A, A representative photomicrograph viewed with a dual fluorescein–rhodamine filter, demonstrating the presence of numerous BrdU⁺ cells in the SVZ and the absence of BrdU⁺ cells in the ependyma lining the lateral ventricle. The arrow designates a double-labeled BrdU⁺/TuJ1⁺ cell with the typical bipolar morphology of a migrating neuron.B–E, Representative photomicrographs of the striatal parenchyma visualized by confocal microscopy and viewed with either a dual fluorescein–rhodamine filter (B,D) or with only a fluorescein filter (C,E). In B and C, the two large cells, with morphology typical of striatal neurons (e.g.,arrowheads), display prominent cytoplasmic TuJ1 staining surrounding their nucleus. The lower cell (arrows) is double-labeled (BrdU⁺/TuJ1⁺). Several cells in the striatal parenchyma adjacent to the subventricular zone, shown in D and E, are BrdU⁺. The neuronal phenotype of one of these cells (arrows) is established by the TuJ1⁺cytoplasm (E) surrounding its nucleus. The upper BrdU⁺ cells (arrowheads) also colocalize TuJ1, but because of the plane of focus, the TuJ1 staining is not limited to the periphery of the nuclei, and therefore their neuronal phenotype cannot be definitively established.F, G, Representative photomicrographs of the septal parenchyma viewed with a dual fluorescein–rhodamine filter (F) or with a fluorescein filter (G). The BrdU⁺/TuJ1⁺ newly generated neuron (arrows) is flanked by numerous TuJ1⁺ fibers (e.g., arrowheads).H–K, Representative photomicrographs of the hypothalamic parenchyma visualized by confocal (H,I) or conventional (J,K) microscopy and viewed with either a dual fluorescein–rhodamine filter (H,J) or with only a fluorescein filter (I, K). In H andI, two cells with a neuronal morphology display prominent MAP-2 staining of their somata and proximal processes (e.g.,arrowheads). The lower cell (arrows) is a double-labeled (BrdU⁺/MAP-2⁺) neuron. In J and K, one of the MAP-2⁺ hypothalamic neurons is also BrdU⁺. ep, Ependyma;LV, lateral ventricle; ST, striatum;SVZ, subventricular zone. Scale bars: A, 25 μm; B, C, 10 μm; D,E, 10 μm; F, G, 10 μm;H, I, 10 μm; J,K, 10 μm.