Table 4.
Free-running behavior of different genotypes during days 3–9 under constant conditions
| Line | Genotype | N | tau | % R4-a | SNR | Sig.4-b | Events/bin | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Standards | 1 | WT | 107 | 23.7 ± 0.1 (91) | 80 | 1.25 ± 0.1 | 4-160 | 17.4 ± 1 | |||||
| 2 | y w; | ; | pdf01 | 45 | 25.4 ± 2 (7) | 16 | 0.50 ± 0.1 | NS | 12 ± 1 | ||||
| 3 | w, | per01 | 29 | 23.0 ± 7.5 (2) | 7 | 0.25 ± 0.2 | 4-160 | 15.3 ± 1 | |||||
| Controls | 4 | y w; | + /UAS-PHM, ; | tim(#16)-gal4 | 14 | 24.1 ± 0.1 (14) | 100 | 0.81 ± 0.2 | 4-160 | 12.4 ± 2 | |||
| 5 | y w; | c929-gal4 | /UAS-PHM | 31 | 24.0 ± 0.1 (23) | 75 | 1.16 ± 0.1 | 4-160 | 20.4 ± 1 | ||||
| 6 | y w; | c929-gal4 | /UAS-PHM,PHM02 | 25 | 23.8 ± 0.1 (15) | 61 | 0.95 ± 0.1 | 4-160 | 22.6 ± 1 | ||||
| 7 | y w; | c929-gal4, | PHM01/y+, CyO | 37 | 23.9 ± 0.1 (27) | 73 | 0.68 ± 0.1 | 4-160 | 16.9 ± 1 | ||||
| 8 | y w; | c929-gal4, | PHM01/UAS-PHM | 28 | 23.6 ± 0.1 (21) | 100 | 1.11 ± 0.1 | 4-160 | 16.1 ± 1 | ||||
| 9 | y w; | y+, | CyO/UAS-PHM,PHM02 | 50 | 23.9 ± 0.3 (41) | 82 | 0.84 ± 0.1 | 4-160 | 16 ± 1 | ||||
| Singlegal4s | 10 | y w; | PHM01/UAS-PHM,PHM02; | 386Y-gal4 | / + | 44 | 24.0 ± 0.4 (37) | 77 | 0.95 ± 0.1 | 4-160 | 8.3 ± 1 | ||
| 11 | y w; | PHM01/UAS-PHM,PHM02; | 36Y-gal4 | / + | 49 | 25.8 ± 0.6 (3) | 6 | 0.29 ± 0.1 | 4-160 | 6.3 ± 1 | |||
| 12 | y w; | c929-gal4, | PHM01/UAS-PHM,PHM02 | 54 | 35.7 (1) | 3 | 0.48 ± 0.1 | — | 19.4 ± 1 | ||||
| Double gal4s | 13 | y w, | pdf-gal4(N); | c929-gal4, | PHM01/UAS-PHM,PHM02 | 60 | 24.5 ± 0.1 (26) | 43 | 0.55 ± 0.1 | NS | 17.5 ± 1 | ||
| 14 | y w, | pdf-gal4(M); | c929-gal4, | PHM01/UAS-PHM,PHM02 | 82 | 23.8 ± 0.2 (18) | 21 | 0.45 ± 0.1 | NS | 11.9 ± 1 | |||
| 15 | y w; | c929-gal4, | PHM01/UAS-PHM,PHM02; | 36Y-gal4 | / + | 23 | 26.8 ± 3.3 (5) | 30 | 0.42 ± 0.1 | NS | 11.8 ± 1 | ||
| 16 | y w; | c929-gal4, | PHM01/UAS-PHM,PHM02; | 386Y-gal4 | / + | 81 | 23.7 ± 0.1 (66) | 83 | 0.71 ± 0.1 | 4-160 | 13.1 ± 1 | ||
| 17 | y w; | c155-gal4; | c929-gal4, | PHM01/UAS-PHM,PHM02 | 34 | 23.4 ± 0.1 (32) | 72 | 0.48 ± 0.1 | NS | 11.4 ± 1 | |||
| 18 | y w, | c929-gal4, | PHM01/UAS-PHM,PHM02; | D42-gal4 | / + | 38 | 23.7 ± 0.3 (20) | 53 | 0.35 ± 0.1 | NS | 10.5 ± 1 | ||
| 19 | y w; | c929-gal4, | PHM01/UAS-PHM,PHM02; | Appl (3GK)-gal4 | / + | 44 | 23.7 ± 0.4 (21) | 48 | 0.42 ± 0.1 | NS | 14.6 ± 1 | ||
| 20 | y w; | c929-gal4, | PHM01/UAS-PHM,PHM02; | tim(#16)-gal4 | / + | 38 | 24.1 ± 0.1 (32) | 84 | 0.76 ± 0.1 | 4-160 | 13.2 ± 1 | ||
| 21 | y w; | PHM01/UAS-PHM,PHM02; | 36Y-gal4 | /tim(#16)-gal4 | 29 | 25.1 ± 0.3 (25) | 84 | 0.89 ± 0.1 | 4-160 | 9.5 ± 1 | |||
tau, Period of the behavioral rhythm in hours. Dunnett'spost hoc analysis, comparing all values to that of line 12 (yw; c929-gal4/UAS-PHM,PHM02) produced the following conclusions:
F4-160: , these stocks different at p < 0.01; NS, these stocks not significantly different. For testing, line 12 was compared separately to each of the four groups (Standards, Controls, Single gal4s, Double gal4s).
F4-a
Percentage rhythmic by χ-square periodogram analysis: p ≥ 10 and w ≥ 2.
F4-b
Significance: by ANOVA, SNR averages are significantly different (p < 0.001).