Fig. 4.

Strategy used to induce adult neuronal recruitment. A, Delivery: schematic coronal section showing site of injection of adenovirus into the lateral ventricle.B, Vector: E1-deleted (ΔE1) adenoviral type 5 constructs used to express a bicistronic transcript of BDNF and hGFP (or hGFP alone, as a control vector) under the control of the constitutive CMV early promoter. C, Experimental protocol: adenovirus was injected on day 1, followed by intraperitoneal injections of 100 mg/kg BrdU for the next 18 d. On day 20, CSF was extracted for BDNF ELISA, and the brains were processed for BrdU immunohistochemistry in tandem with phenotype-specific immunolabeling.