Fig 2. Topo II inhibition and interaction with Ptox^Pdp.

(A) Topo II inhibition by Ptox^Pdp. Line 1, pUC18; line 2, pUC18 plus nuclear extract; line 3, pUC18 plus nuclear extract and etoposide (50 μM); line 4, pUC18 plus nuclear extract and 0.75 μM Ptox^Pdp; line 5, pUC18 plus nuclear extract and 1.5 μM Ptox^Pdp. (B) Ptox^Pdp docked to the Topo II-DNA complex. (C) Interaction between Ptox^Pdp and nearby residues. CL = cleaved; L = linear; S = supercoiled; R = relaxed DNA. Unk0 = Ptox^Pdp. Dark green dash lines indicate hydrogen bonds. The eye-like curved dark red lines represent the hydrophobic residues in contact.