Fig 4. Inhibition of liver cancer cell migration and invasion by Ptox^Pdp.

(A) Inhibition of HCCLM3 cells migration by Ptox^Pdp. (B) Inhibition of HCCLM3 cell invasion by Ptox^Pdp and quantification. The invasive cells were stained with crystal violet. The results were expressed as invasive cell numbers per field of view (mean± 5 SD, n = 6). ***P < 0.001 compared with DMSO-treated group. (C) Wounded HCCLM3 cells were treated with 0, 0.75, or 1.50 μM Ptox^Pdp for 18 h. (D) Quantitative statistics analysis of the width of gaps in the wound assay in (C). ***P < 0.001 compared with DMSO-treated group. (E) Western blotting (top) and gelatin zymography (bottom) analyses of matrix metalloprotease inhibition, under the indicated conditions. The object size: 10×10 in Fig 4A and 20×10 in Fig 4C.