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RP-HPLC fractionation of WT and PC2-deficient mouse hypothalamic extracts after gel exclusion, as in Figure 3. The pooled extracts of β-endorphin-sized material were fractionated on the gradient indicated, and the fractions were assayed for β-endorphin immunoreactivity. The arrows indicate the elution times of authentic β-endorphin peptides. The dashed line indicates the acetonitrile gradient. Similar results were seen in two experiments. Ac, Acetyl.
