Fig. 6.

Sephadex G-50 chromatography of WT and PC2 mutant mouse hypothalamic extracts. Gel filtration was performed as described in Materials and Methods. Portions of the fractions were vacuum dried and assayed for OFQ/N immunoreactivity. ¹²⁵I-OFQ/N elutes at fraction 55. Cytochrome C (molecular mass, 22.5 kDa) elutes at fraction 33–34. V₀, Exclusion volume; V_t, total column volume. Conversion of total OFQ/N immunoreactivity to mature peptide in WT and PC2 mutant mouse hypothalamus is shown in the inset. The percentage of conversion is the ratio of the immunoreactivity co-eluting with synthetic OFQ/N at 31 min to the total OFQ/N immunoreactivity. We used RP-HPLC analyses for this calculation.n = 3; p = 0.002; unpairedt test using StatView.