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RP-HPLC fractionation of WT and PC2 mutant amygdala extracts. Extracts from WT animals (open circles) and PC2 mutant animals (filled circles) were fractionated using a linear gradient of 8–36% acetonitrile (dashed line). The fractions were assayed for OFQ/N immunoreactivity. The arrow indicates the elution time of synthetic OFQ/N. The profile shown is representative of four separate determinations.
