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. 2001 Aug 15;21(16):6086–6094. doi: 10.1523/JNEUROSCI.21-16-06086.2001

Fig. 5.

Fig. 5.

Suppression of neuronal cell death in the piriform cortex and amygdala by CDK4 or cyclin D1 antisense oligonucleotides.A, 5′-Amino-3′-biotin-labeled oligonucleotide was detected in cells around the ipsilateral but not the contralateral side of the lateral ventricle in the rat brain 2.5 d after the start of infusion. Staining was mainly found in the ependymal cells and presumably astrocytes. B, C, CDK4 immunoreactivity was suppressed by CDK4 antisense oligonucleotide (B;green), and cyclin D1 immunoreactivity was suppressed by cyclin D1 oligonucleotide (C; green) 1 d after KA administration. Suppression was observed in the ipsilateral but not the contralateral side of the piriform cortex. NeuN staining in the identical fields is also shown (B, C;red). D, E, The number of TUNEL-positive cells was dramatically reduced in the ipsilateral but not contralateral piriform cortex and amygdala by infusion of CDK4 (D) or cyclin D1 (E) antisense oligonucleotide 2 d after KA administration. Sections were counterstained with neutral red. F, G, Immunostaining of c-Fos in the piriform cortex and amygdala 12 hr after KA administration is shown. No apparent change was observed by infusion of CDK4 (F) or cyclin D1 (G) antisense oligonucleotide. CL, Contralateral; IL, ipsilateral; LV, lateral ventricle. Scale bars: A, D–G, 200 μm;B, C, 100 μm.