Fig. 1.

mRNA encoding SERT can be demonstrated in the murine gut by means of RT-PCR. The expected PCR product is 357 bp. mRNA encoding SERT is not found in the brains (positive control) or in the colons of SERT −/− mice. In contrast, in wild-type (SERT +/+) mice, both the brain and colon contain mRNA encoding SERT. Thelanes marked + illustrate the results obtained when RT was present. The lanes marked− were obtained when RT was absent (control indicating that the preparations were not contaminated with DNA).