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. 2001 Aug 15;21(16):6348–6361. doi: 10.1523/JNEUROSCI.21-16-06348.2001

Fig. 5.

Fig. 5.

mRNA encoding OCT-1 and OCT-3 can be demonstrated in the murine gut by means of RT-PCR. The expected PCR products are 697 bp for OCT-1 and 568 bp for OCT-3. A, mRNA encoding OCT-1 is found in both the brains and colons of SERT −/− mice. In contrast, only the colon and not the brain contains mRNA encoding OCT-1 in wild-type (SERT +/+) mice. mRNA encoding β-actin was simultaneously demonstrated by RT-PCR. B, mRNA encoding OCT-1 is found in both the small intestines and the colons of SERT +/+, +/−, and −/− mice. Expression of OCT-1 appears to be greater relative to that of β-actin in the small intestines of SERT −/− mice compared with the small intestines of SERT +/− or +/+ mice. A similar apparent upregulation of OCT-1 is not seen in the SERT −/− colon. C, mRNA encoding OCT-1 appears to be upregulated in the small intestines of SERT −/− mice, but the knock-out of DAT does not appear to affect the expression of OCT-1. D, mRNA encoding OCT-3 is found in both the small intestines and the colons of SERT +/+, +/−, and −/− mice. The knock-out of DAT does not affect the expression of OCT-3.