Fig. 5.

Inhibition of Schwann cell adhesion and spreading on collagen type IV and α4(V) NTD. A, B, Schwann cells were plated on dishes coated with collagen type IV or α4(V) NTD and incubated for 3 hr in serum-free medium with heregulin alone or in medium with the anti-α1-integrin antibody (α1), the anti-α2-integrin antibody (α2), both antibodies (α1+2), the anti-β1-integrin antibody (β1), a control hamster monoclonal antibody (con Ab), or 10 μg/ml heparin (hep). Adhesion was measured by the crystal violet assay. Values that were significantly different from controls are indicated (*p < 0.05; **p < 0.01).C, Schwann cells were plated on dishes coated with collagen type IV (col IV) or the α4(V) NTD and incubated for 24 hr in serum-free medium with heregulin alone or in medium with the anti-α1-integrin antibody (α1), the anti-α2 integrin antibody (α2), a control hamster monoclonal antibody (con Ab), or 10 μg/ml heparin (hep). Schwann cell spreading was monitored by phase contrast microscopy. The micrographs from the left andrightsets of panels were from different experiments.