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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Kidney Int. 2019 Feb 21;95(6):1347–1358. doi: 10.1016/j.kint.2018.11.027

Figure 3. FSP-1+ cells express cytokines of type I macrophages in AVFs with CKD.

Figure 3.

A & B. Serum BUN and creatinine were carried out in control and CKD mice. (n = 6, *, p <0.05, vs control). C & D. CKD increases VSMC dedifferentiation. The expression of VSMC markers were determined by immunostaining (C) and RT-PCR (D) in the 2 week AVFs that were created in VSMCGFP mice after sham and CKD. Representative images and RT-PCR results were shown (n = 6 mice; mean ± SD,* p < 0.05 compared with artery; #, p < 0.05, compared with AVF control). E. AVF were created in control and CKD mice and collected after 2 weeks, co-immunostaining of FSP-1 and type I macrophages markers, iNOS, were performed (left panel). Statistical analysis of FSP-1+/iNOS+ cell numbers is shown in right panel. F. Arrays of cytokines in the AVFs from control or CKD mice were detected by real-time RT-RCR, indicating that cytokines from macrophage type 1 were induced by CKD. (n = 6; mean ± SD; * p < 0.05 compared with AVF control). Scale bar = 50 μm in all panels.