Table 3.

Disruption temperatures used in papers compiled in this review. Note that some papers use multiple temperatures so percentages may not add to 100%. RT denotes room temperature and varies considerably between publications. Not specified is probably room temperature by default.

MODE OF MEMBRANE DISRUPTION:	37 °C	RT (~18 – 25 °C)	< 4 °C	Not Specified
ALL	22% (n=65)	46% (n=139)	16% (n=47)	16% (n=49)
Electroporation	9% (n=15)	67% (n=112)	11% (n=19)	13% (n=22)
Physical (non-electroporation)	34% (n=30)	25% (n=22)	12% (n=11)	27% (n=24)
Biochemical	43% (n=20)	13% (n=6)	38% (n=18)	6% (n=3)