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. 2019 Sep 9;129(10):4332–4349. doi: 10.1172/JCI121955

Figure 11. YAP/TAZ suppress β-catenin– and NICD-mediated DLL4 induction.

Figure 11

(A–C) YAP/TAZ siRNA sensitized HUVECs to Akt activation in response to 50 ng/mL VEGF-A (A) and 400 ng/mL Ang-1 (B), and 10% FBS (C) for 10 minutes. (D and E) DAPT (10 μM, 3 hours) or MK2206 (10 μM, 3 hours) abolished YAP/TAZ siRNA–induced mRNA (D) and protein (E) expression of DLL4. (F and G) DAPT (10 μM, 10 minutes pretreatment) or MK2206 (10 μM, 10 minutes pretreatment) attenuated mRNA (F) and protein (G) expression of DLL4 induced by Y27632 (10 μM, 3 hours). (H and I) β-Catenin siRNA abolished YAP/TAZ siRNA–induced mRNA (H) and protein (I) expression of DLL4. Data are mean ± SEM of triplicates. **P < 0.01, ***P < 0.001, 1-way ANOVA followed by Tukey’s multiple-comparisons test. Unprocessed original scans of Western blots are shown in Supplemental Figure 15.