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. 2019 Sep 4;129(10):4316–4331. doi: 10.1172/JCI129317

Figure 7. VC stimulates TET activity to promote Th1-type chemokine and PD-L1 expression.

Figure 7

(A) VC increased TET activity in cultured cells. Control and TET2-KO THP-1 cells or B16-OVA cells were treated with l-ascorbic acid (L-AA; 250 μM) as indicated for 24 hours, total genomic DNA was extracted, and 5hmC level was determined by dot blot and quantified. Error bars represent ± SD for triplicate dilutions. (B) VC increased IFN-γ–induced chemokine and PD-L1 gene expression in THP-1 cells. Control and TET2-KO THP-1 cells were treated with IFN-γ and L-AA (250 μM) as indicated, and total RNA was extracted. mRNA levels of chemokines and PD-L1 were determined by qPCR. UD, undetectable. Error bars represent ± SD for triplicate experiments. (C) VC enhanced IFN-γ–induced chemokines and Pdl1 expression in B16-OVA cells. Control and Tet2-KO B16-OVA cells were treated with IFN-γ and L-AA (250 μM) as indicated, and total RNA was extracted. mRNA levels of chemokines and Pdl1 were determined by qPCR. Error bars represent ± SD for triplicate experiments.