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. 2019 Oct 1;129(10):4022–4031. doi: 10.1172/JCI129191

Figure 2. New strategies to isolate adipose tissue–resident progenitor subpopulations from murine gonadal adipose tissue.

Figure 2

(A) Gonadal WAT depots of adult mice harbor functionally distinct PDGFRβ+ subpopulations. Molecular markers, including cell surface markers, are shown. The PDGFRβ+LY6C–CD9– fraction represents highly adipogenic adipocyte precursor cells (APCs) enriched in the expression of Pparg. These cells differentiate spontaneously upon reaching confluence in vitro when maintained in media containing insulin. The PDGFRβ+LY6C+ fraction represents fibro-inflammatory progenitors (FIPs). These cells are largely refractory to adipogenic stimuli and can actually be anti-adipogenic. (B) FACS strategy to isolate PDGFRβ+ subpopulations from perigonadal WAT of adult mice. Following the depletion of endothelial (CD31+) and hematopoietic cells (CD45+) from the stromal vascular fraction of digested adipose tissue, PDGFRβ+ cells can be subdivided into APCs and FIPs on the basis of LY6C and CD9 expression.