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. 2019 Sep 4;129(10):4224–4238. doi: 10.1172/JCI127458

Figure 7. Overexpression of Serpinb9 in Ifnar1-KO cancer cells reduces enhanced killing by CD8+ T cells in vitro.

Figure 7

WT and Ifnar1-KO MC38 cells were cocultured with CD8+ T cells isolated from WT MC38 tumors for 48 hours with or without Z-AAD-CMK. CD8+ T cells were treated with Z-AAD-CMK (100 μM) for 30 minutes prior to coculture. Z-AAD-CMK remained in the medium through the experiment. (A) Percentage of cell killing. n = 4. MC38 cells (WT, Ifnar1-KO + vector, and Ifnar1-KO + Serpinb9 overexpression [SB9 OE]) were cocultured with CD8+ T cells isolated from WT MC38 tumors. (B) Percentage of cell killing. n = 4. MC38 cells (WT, Ifnar1-KO + vector, and Ifnar1-KO + SB9 OE) were cocultured with either control medium or CD8+ T cells isolated from WT MC38 tumors for 4 hours with propidium iodide (50 μg/mL) in the medium. (C) Percentage of propidium iodide–positive cells following coculture. n = 4. Data represent mean ± SD. Comparison of means was performed by 1-way ANOVA with Tukey’s multiple-comparisons test (NS: P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001).