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. 2019 Aug 26;129(10):4110–4123. doi: 10.1172/JCI125963

Figure 6. CHIP degrades MAST1, which consequently sensitizes cisplatin-resistant cells to cisplatin.

Figure 6

(A) Effect of CHIP overexpression and MAST1 knockdown on cisplatin sensitivity and MAST1 protein level. (B) Effect of MAST1 WT rescue expression on cisplatin sensitivity and MAST1 protein level in cells with CHIP overexpression and MAST1 knockdown. (C) Effect of CHIP and MAST1 WT or 2KR overexpression on cisplatin sensitivity and MAST1 protein level. KB-3-1cisR and A549cisR cells with flag-CHIP and MAST1 knockdown or WT/2KR overexpression were treated with increasing concentrations of cisplatin in the presence of 17-AAG for 48 hours. Cell viability was determined by CellTiter-Glo assay. Data are mean ± SD from 3 technical replicates and representative of 4 (A) and 2 (B and C) independent biological experiments. Statistical analysis was performed by 1-way ANOVA. ***P < 0.005; ****P < 0.0001.