Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal anti-FLAG M2 | Sigma-Aldrich | Cat# F1804 RRID:AB_262044 | (1:1000) |
| Antibody | Rabbit polyclonal anti-pan Neurexin-1 | Millipore | Cat# ABN161 RRID:AB_10917110 | (1:1000) |
| Antibody | Mouse monoclonal anti-HA1.1 | Bioledgend | Cat# 901513, RRID:AB_2565335 | (1:5000) |
| Antibody | Mouse monoclonal anti-Vinculin | Sigma-Aldrich | Cat# V9131, RRID:AB_477629 | (1:10000) |
| Antibody | Rabbit polyclonal anti-GABAARα1 | Synaptic System | Cat# 224 203, RRID:AB_2232180 | (1:1000) |
| Antibody | Rabbit polyclonal anti-GABAARα6 | Synaptic System | Cat# 224 603, RRID:AB_2619945 | (1:1000) |
| Antibody | Mouse monoclonal anti-Vglut1 | Millipore | Cat# MAB5502, RRID:AB_262185 |
(1:1000) |
| Antibody | Guinea pig polyclonal anti-vGAT | Frontier Institute co. Ltd | Cat#: VGAT-GP-Af1000, RRID: AB_2571624 | (1:1000) |
| Antibody | Mouse monoclonal anti-beta Actin | Abcam | Cat#: ab20272 RRID:AB_445482 |
(1:2000) |
| Antibody | Rabbit polyclonal anti-TGF beta 1 | Abcam | Cat#: ab92486, RRID:AB_10562492 | (1:1000) |
| Antibody | Rabbit polyclonal anti-MeCP2 | Zoghbi Lab | #0535 | (1:1000) |
| Antibody | Mouse monoclonal anti-Gephyrin | Synaptic system | Cat#: 14701, RRID:AB_887717 |
(1:1000) |
| Antibody | Rabbit polyclonal anti- PSD-95 |
Cell signaling | Cat # 2507, RRID:AB_561221 |
(1:1000) |
| Recombinant DNA reagent | pLenti-mCherry | Addgene | Cat# 36084, RRID:Addgene_36084 |
|
| Recombinant DNA reagent | pLenti-Nxph4-3xFLAG-mCherry | This paper | N/A | Results subsection‘Nxph4 is a secreted glycoprotein’ |
| Recombinant DNA reagent | pLenti--Nxph4-4Q-HA-mCherry | This paper | N/A | Results subsection ‘Nxph4 is a secreted glycoprotein’ |
| Recombinant DNA reagent | pLenti--Nxph4-6A-HA-mCherry | This paper | N/A | Results subsection ‘Nxph4 is a secreted glycoprotein’ |
| Recombinant DNA reagent | pCMV-Nxph4-HA | This paper | N/A | Results subsection‘Nxph4 is a secreted glycoprotein’ |
| Recombinant DNA reagent | pCMV-Nxph4-4Q-HA | This paper | N/A | Results subsection‘Nxph4 is a secreted glycoprotein’ |
| Recombinant DNA reagent | pCMV-Nxph4-6A-HA | This paper | N/A | Results subsection‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCAG-HA-Nrxn1β S4(+) | Addgene | Cat# 59410, RRID:Addgene_59410 |
|
| Recombinant DNA reagent | pCAG-HA-Nrxn1β S4(-) | Addgene | Cat# 59409, RRID:Addgene_59409 |
|
| Recombinant DNA reagent | pCAG-HA-Nrxn1α | Addgene | Cat# 58266, RRID:Addgene_58266 |
|
| Recombinant DNA reagent | pCAG-HA-Nrxn1α-LNS1 | This paper | N/A | Results subsection‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCAG-HA-Nrxn1α-LNS2 | This paper | N/A | Results subsection‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCAG-HA-Nrxn1α-LNS3 | This paper | N/A | Results subsection‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCAG-HA-Nrxn1α-LNS4 | This paper | N/A | Results subsection‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCAG-HA-Nrxn1α-LNS5 | This paper | N/A | Results subsection ‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCMV-GABARα1N-HA | This paper | N/A | Results subsection ‘Nxph4 interacts with α-neurexin in vivo’ |
| Recombinant DNA reagent | pCMV-GABARα6N-HA | This paper | N/A | Results subsection ‘Nxph4 interacts with α-neurexin in vivo’ |
| Chemical compound, drug | X-Gal | Thermo Fisher | Cat# 15520034 | |
| Chemical compound, drug | Lipofectamine 2000 | Thermo Fisher | Cat# 11668500 | |
| Commercial assay, kit | Antigen Unmasking Solution (citrate based) | Vector Laboratories | Cat# H-3300 | |
| Commercial assay, kit | Avidin/Biotin Blocking Kit |
Vector Laboratories | Cat# SP-2001 | |
| Commercial assay, kit | TSA Kit | Invitrogen | Cat# T-20932 | |
| Commercial assay, kit | Papain Dissociation System Kit | Worthington Biochemical Corp. | Cat# LK003150 | |
| Commercial assay, kit | PNGase F | NEB | Cat# P0704S | |
| Commercial assay, kit | miRNeasy Mini Kit | Qiagen | Cat#: 217004 | |
| Commercial assay, kit | M-MLV Reverse Transcriptase | Thermo Fisher | Cat#: 28025013 | |
| Commercial assay, kit | iTaq Universal SYBR Green SuperMix | BIO-RAD | Cat#: 1725124 | |
| Cell line (Homo sapiens) | HEK293T cells | ATCC | RRID:CVCL_0063 | |
| Strain, strain background (Mus musculus) |
Mouse: FVB | The Jackson Laboratory | Stock No: 001800 | |
| Strain, strain background (Mus musculus) |
Mouse: Nxph4 βgeo+/- | This paper | N/A | The Jackson Laboratory: 033791 |
| Strain, strain background (Mus musculus) |
Mouse: Nxph4 FLAG/FLAG | This paper | N/A | The Jackson Laboratory: 033792 |
| Sequence-based reagent | Nxph4βgeo+/- genotyping forward primer: 5’-AAAGACTAGCAGACGCAGCA | This paper | N/A | Materials and methods subsection ‘Nxph4-βgeo mouse’ |
| Sequence-based reagent | Nxph4βgeo+/- genotyping reverse primer: 3’-CCCTAACTCCCCCAAACAGA | This paper | N/A | Materials andmethods subsection ‘Nxph4-βgeo mouse’ |
| Sequence-based reagent | Nxph4 FLAG/FLAGgenotyping forward primer: 5’-TCAAGTTCTCGCTGTTGGTG | This paper | N/A | Materials and methods subsection‘Nxph4FLAG/FLAG knock-in mouse’ |
| Sequence-based reagent | Nxph4 FLAG/FLAG genotypingreverse primer: 3’-TTCCACGTGGCAATTAAAAG | This paper | N/A | Materials and methods subsection‘Nxph4FLAG/FLAG knock-in mouse’ |