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. 2019 Sep 16;129(10):4506–4522. doi: 10.1172/JCI128503

Figure 2. Acute CaOx crystal deposition leads to rapid tubule dilation and activation of PKD-associated signaling pathways in WT C57/BL6 mice.

Figure 2

(A) H&E-stained kidney sections 1 day after administration of 0.7 mg/kg NaOx, visualized by normal and polarized light microscopy. Scale bar: 1 mm. (B) High-magnification images from A, using polarized light. Scale bar: 100 μm. (C) Immunoblot of total kidney lysates for p-S6 (Ser235/236), p-STAT3 (Tyr705), and total proteins 3 hours (n = 4) and 1 day (n = 9) after 0.7 mg/kg NaOx treatment. Immunoblots are representative of 2 experiments. (D) Polarized light micrographs of kidney sections from mice treated with 0.3 mg/kg NaOX, 6 hours (n = 4), 1 day (n = 12), 3 days (n = 8), and 7 days (n = 13) after treatment. Scale bar: 100 μm. Original magnification, ×2 (inset). (E) Immunofluorescence staining of p-S6 (Ser235/236) and p-STAT3 (Tyr705) in mice treated with 0.3 mg/kg NaOx. Images of animals treated with 0.3 mg/kg NaOx are representative of 5 experiments. Scale bar: 50 μm.