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. 2019 Sep 9;129(10):4377–4392. doi: 10.1172/JCI127718

Figure 1. An ex vivo screen identifies compounds that inhibit the growth of metastatic OS.

Figure 1

(A) Outline of the PuMA screen. (B) Left: 96-well plate of lung explants seeded with metastatic OS cells. Center, left: magnified view of 4 individual wells. Fluorescence image of a lung explant seeded with GFP+ OS cells (top center) and corresponding false colored image used for quantification (top right). Bottom center: ×40 magnification of H&E-stained, control-treated lung section after 14 days in PuMA explant culture. Boxed region highlights area of extensive OS cell growth. Bottom right: ×200 magnification of boxed region. (C) Distribution of 112 of the compounds tested, according to class. (D) Fluorescence image of a 96-well plate of lung explants treated for 14 days with 2 doses (high and low) of each compound or vehicle control (DMSO, white boxes). Called hits are boxed in red. Each row (M1–M8) is from a single mouse. (E) Dot plot showing results of all compounds tested at all doses. The dashed line corresponds to 90% reduction of GFP+ area compared with DMSO controls after 14 days of treatment in the PuMA model.