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. 2019 Sep 9;129(10):4377–4392. doi: 10.1172/JCI127718

Figure 4. E9 treatment impairs transcription elongation in OS cells.

Figure 4

(A) Western blot analysis of the indicated proteins in 143B and MG63.3 cells treated with DMSO or increasing concentrations of E9 at 6 and 24 hours. GAPDH, loading control. (B) Representative browser views of ChIP-Seq data at the SLC38A1 locus. (C) Windowed heatmaps showing H3K27ac, CDK12, RNA Pol2, and RNA Pol2 Ser2 ChIP-Seq signals ± 5 kb from the TSS in 143B cell line. Below are aggregate plots showing the respective ChIP-Seq signals for the heatmaps depicted above. (D) Metagene analysis of Ser2 ChIP-Seq signal across all genes sorted in descending order based on the average signal per binned region. Windows include 1 kb upstream of the TSS and 1 kb downstream of the TES. (E) Browser views of Ser2 ChIP-Seq signal at RUNX1 and FOS loci in DMSO- and E9-treated OS cells.