Figure 1:

Model of Rad51-dependent BIR. A. 5’ to 3’ resection of a one-ended DSB generating a 3’ ssDNA end (blue), where the assembly of Rad51 filaments takes place. B. Invasion of the 3’ end containing Rad51 nucleoprotein filament into a homologous chromosome (black), followed by D-loop formation. C. Initiation of BIR DNA synthesis using a homologous chromosome as a template. Nascent DNA represented by dotted lines. D. Progression of DNA synthesis with the migration of the BIR replication bubble, generating a long, persistent ssDNA tail behind the bubble. E. Completion of BIR associated with conservative inheritance of a newly synthesized strand (Donnianni and Symington, 2013, Saini et al., 2013). A color version of the figure is available online.