Fig. 2. Isl1 knocked down efficiency at E12.5 urethra in Isl1-inducible knockout embryos.

a Schematic of inducible knockout mouse. b Isl1 gene knockout strategy and location of primers used for PCR verification of knockout genotype. c Genotype of Isl1^MCM/F mutants and Isl1^F/+ controls assayed by common PCR. d Representative image of Western blot detecting the knockdown efficiency of ISL1 protein. e–e3 ISL1 expression in urethral epithelium of Isl1^MCM/Del and control embryos. Scale bars: e–e1, 50 μm; e2–e3, 10 μm. Red arrowheads representative ISL1-positive cells. Results were presented as means ± SEM. Each test contained at least four independent replications for Western blot and PCR. *P < 0.05