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. 2019 Sep 26;10(10):713. doi: 10.1038/s41419-019-1952-z

Fig. 6. ISL1 binds to the Shh promoter.

Fig. 6

a, b Expression of urethral epithelium candidate genes were compared by qPCR in normal and knockout mice at E12.5 and E15.5 in male GTs. c Dual-Luciferase reporter assays. Only the Shh promoter exhibited a significant increase in relative luciferase activity in response to ISL1. d Yeast one-hybrid assays. BD-ISL1 activated the LacZ reporter gene driven by the Shh promoter fragments (AD-Shh), but not Fgf8 (AD- Fgf8) and Fgfr2 (AD- Fgfr2) promoter fragments. e, f SHH protein levels were examined by Western blot. g SHH expressed in the urethral epithelium at E12.5. The white dotted line marks the boundary of urethral epithelium. Red staining is SHH, and DAPI nuclear counterstaining (DNA) is blue. Scale bars: 20 μm. Results were presented as means ± SEM. At least four independent experiments for qPCR and Dual-Luciferase reporter assays were used. *P < 0.05, **P < 0.01, and ***P < 0.001