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. 2019 Sep 26;9:13931. doi: 10.1038/s41598-019-50329-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of the experimental procedures used in the experiment described in Fig. 8. (A) Untreated control cells (MKN-45, human gastric carcinoma cells) were cultivated in parallel to the other assays. (B,C) MKN-45 cells (125 000 cells/ml) in 24 well tissue culture clusters were treated with CAP for 1 min. After CAP treatment, the cells remained in contact with the same medium for 25 min. 100 µM of the NOX inhibitor AEBSF was present during CAP treatment and incubation in assay C. Assays were washed after the 25 min incubation step and were further cultivated in fresh medium. (D,E) Medium was treated with CAP for 1 min in the absence of cells and was then transferred to cells. The cells in assay (E) were washed after the incubation step and further cultivated in fresh medium. (F,G) Cells were treated with CAP for 1 min and then washed immediately. In assay (G), 100 µM AEBSF was present during CAP treatment. In all assays, the percentages of apoptotic cells were determined at 4, 6 and 7.5 h.