Figure 4.

Basic scheme of the experiments described in Figs 9–13. (A) Untreated MKN-45 cells (control). (B,C) MKN-45 cells were treated with CAP for 1 min. The cells were further incubated in the same medium for additional 25 minutes and then washed (3 cycles) and resuspended in fresh medium. Inhibitors (INH) (as indicated in the respective figures) were present during CAP treatment and the incubation step. In the assays described under C, 100 µM AEBSF was present in all assays (for prevention of the generation of secondary ¹O₂), together without or with additional inhibitors, as indicated. Inhibitors were also added after the washing step, where indicated. (D) Cells were treated with CAP for 1 min, in the absence or presence of the indicated inhibitors and were then washed immediately and resuspended in fresh medium. Where indicated, inhibitors were added and cultivation was continued. The percentages of apoptotic cells were determined kinetically.