Table 1.

Thermodynamic and kinetic data of folding of L6-NTD compared to WT-NTD

	WT-NTDa	L6-NTD
mD-NCD (kcal mol−1 M−1)	1.69 ± 0.03	1.30 ± 0.05
mD-NF (kcal mol−1 M−1)	1.69 ± 0.02	1.37 ± 0.03
[urea]50%CD (M)	3.30 ± 0.01	5.99 ± 0.02
[urea]50%F (M)	3.31 ± 0.01	5.95 ± 0.01
ΔGD-NCD (kcal mol−1)	5.6 ± 0.1	7.8 ± 0.3
ΔGD-NF (kcal mol−1)	5.6 ± 0.1	8.2 ± 0.2
kf (s−1)	13,000 ± 6000	519,000 ± 213,000
ku (s−1)	3 ± 1	0.2 ± 0.1
mf (kcal mol−1 M−1)	1.2 ± 0.1	1.13 ± 0.05
mu (kcal mol−1 M−1)	0.23 ± 0.03	0.30 ± 0.04
β T	0.84 ± 0.02	0.79 ± 0.09

β_T denotes the Tanford β-value, calculated from m_f and m_u (ref. ¹⁷). Errors of β_T are propagated s.e. from regression analysis

^aEquilibrium thermodynamic quantities are from ref. ¹⁷; kinetic quantities are from ref. ¹¹. Errors are s.e. from regression analysis