Fig. 3.

Confocal LNA-PRISM images of rat hippocampal neuronal synapses. a 13-channel images of 21 days in vitro (DIV) rat hippocampal neuronal culture from a single field of view. Individual channels for each marker are shown followed by a composite image in the bottom-right corner. MAP2 and VGLUT1 were visualized using fluorescently labeled secondary antibodies, nuclei were visualized using DAPI, and all other targets were visualized using ssLNA imaging probes. Synapsin-I was imaged twice, once in the middle and once at the end of the experiment. b Zoom-in view of a single dendrite indicated by the white box in a. Scale bars: a 20 μm; b 2 μm