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. 2019 Sep 26;10:4377. doi: 10.1038/s41467-019-12372-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Super-resolution DNA-PRISM imaging of primary neuronal cultures. a Widefield and DNA-PRISM images of neuronal microtubules stained using the DNA-conjugated anti-Tuj-1 antibody. b Zoom-in view of the boxed areas in (a) show resolution enhancement of DNA-PRISM images compared with widefield images. The arrowhead indicates distinct microtubule bundles that are not resolved in the widefield image. c Widefield and DNA-PRISM images of filamentous actin stained using DNA-conjugated phalloidin. d Zoom-in views of the boxed areas in c show two actin filaments (left) and the synaptic actin punctae with sub-synaptic structures (right, arrowhead) that are not resolved in widefield images. e Widefield and DNA-PRISM images of pre-synaptic marker synapsin-I (red) and post-synaptic marker PSD-95 (cyan) of the same field of view. f Zoom-in view of single synapses indicated by boxes in e. g Cross-sectional profile of the boxed region in (b) shows a microtubule bundle next to a possible single microtubule with FWHM = 47 nm. h Cross-sectional profile of the boxed region in d shows two actin filaments or small filament bundles that are 80 nm apart. i The average size of synapses defined by synapsin-I and PSD-95 is quantified using the normalized radial cross-correlation function. The decay at the smaller radial shift of the DNA-PRISM curve (red) indicates the smaller synapse size in the DNA-PRISM image due to the improved spatial resolution. Scale bar: a, c, e 10 μm; b, d, f 0.5 μm