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. 2019 Sep 26;9:13943. doi: 10.1038/s41598-019-50208-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence data from cell kinetic studies for 0, .5, 1, 2, 4, 6, 12, and 24 hours. Total fluorescence for wells with (CSI) and without (MPE) cells exposed to 10 nM for (a) QSH and (b) PS. Fluorescence intensities I_CSI,t and I_MPE,t for (a) differ significantly (P < 0.05) for QSH at approximately 4 hours, indicating cell-induced cellular induced degradative effects. Fluorescence intensities I_MPE,t and I_MPE,0 differ significantly from 30 minutes, indicating media degradation at this time. For PS (b), no significant cell induced degradation is present (P > 0.05). Media induced degradation begins at 6 hours (P < 0.05). Differences between I_CSI,t and I_MPE,t and I_MPE,t with I_MPE,0 were plotted in (b), showing a steady increase for QSH and constant intensity for PS. Media (I_mdeg) and cell (I_cdeg) induced degradative values were calculated from (a, b) and plotted against time in (c). Fluorescence intensities were taken after washing 2X with complete growth medium and further trypsinization for (d) QSH and (e) PS. Cell fluorescence values from CKD (d,e) were ally used to determine concentration (nM) of QSH and PS NM uptake/adsorption in (f). The calibrated concentration of QSH (black, solid) shows significantly higher values (P < 0.05) than raw values (black, dashed). However, PS does not degrade, so calibrated (blue, solid) and raw (blue, dashed) concentrations are not significantly different. Statistical t-test p-values are described as follows: ***P < 0.001, **P < .01, and *P < 0.05.