Fig. 1. UM0112176 inhibits proliferation of cancer cells while stimulating cytoskeleton disruption and caspase activation.

a The quantity of normal and cancer cells observed when incubated for 48 h in the presence or absence of the 10 μM UM0112176 and normalized with respect to t = 0. The values are given as a mean and SD, *p < 0.05 for all normal and cancer cell lines. b The quantity of normal and cancer cells surviving after 7-day treatment with 10 μM UM0112176. The values are given as a mean and SD, *p < 0.05. c The effect of 10 μM UM0112176 (24 h of treatment) on Ki67 expression in KLN205 and astrocytes. Expression of Ki67 in cells growing in the absence of the inhibitor was normalized to be 100%. The values are given as a mean and SD, *p < 0.05. d The activation of caspase 3 in KLN205 cells after treatment with UM0112176 (10 μM, 24 h). Bar = 20 µm. The graph below shows the number of cells [in %] exhibiting active caspase 3 fluorescence. e The effect of UM0112176 on the morphology of the actin cytoskeleton in cancer cell line, KLN205 and hNSCLC, and in normal cells, astrocytes and fibroblasts. Bar = 10 µm