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. 2019 Sep 26;9:13923. doi: 10.1038/s41598-019-50385-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Excitation (dashed) and emission (solid) spectra of unreacted and TCE-reacted BSA. Fluorescence intensity (FI) of 0–1 µg/µL BSA following 5 minutes of UV-exposure in the (A) absence or (B) presence of 0.5% (v/v) TCE. Emission spectra (emission ʎ = 350–600 nm; excitation ʎ = 310 nm) and excitation spectra (emission ʎ = 450 nm; excitation ʎ = 250–400 nm) were monitored in a BioTek Cytation 5 microplate reader.