Fig. 6. Linc00645 modulates TGF-β-induced glioma cell migration and invasion via miR-205-3p.

a, b Glioma cells were transfected with miR-205-3p mimics/NC or miR-205-3p inhibitor/inhibitor NC and subsequently treated with TGF-β. The lin0065 level were determined by qRT-PCR. c, d Western blot analysis showed the expression of E-cadherin, Vimentin, N-cadherin, and ZEB1 in miR-205-3p inhibitors and si-Linc 1# transfected U251 and T98G cells. The protein level was displayed with bar graph. e, f Wound healing assay and transwell assay in miR-205-3p inhibitors and si-Linc 1# transfected U251 and T98G cells. The invasion and migration rate were displayed with bar graph. Data are shown as the mean ± SD of three independent experiments. (*P < 0.05, **P < 0.01 and ***P < 0.001)