Figure 1.

Representation of the different irak3-transcript lengths. The irak3-variants of trout are listed together with their accession numbers and their tissue-wise occurrence on the left. Tissues are abbreviated as follows: Bo, bone; Br, brain; E, unfertilized eggs; G, gills; HK, head kidney; L, liver; S, spleen; T, thymus. The genomic organization of the trout irak3 gene is drawn above the scheme. Exons are represented by colored boxes (red, death domain; green, ProST region; light blue, pseudokinase domain; dark blue, C-terminal domain). Lines between exons represent introns. A cross indicates that the respective exon is present in the listed transcript variant; if the exon length differs from the prototypic length, the actual length is given (in nt); a superscript p denotes a partial 3′-end. The position where a fragment of intron 8 was inserted in the predicted irak3 variant (3) is marked with a gray arrow. Asterisks indicate the possible usage of alternative splice acceptor/donor sites. The color of the characters denotes a particular protein domain. Fields highlighted in yellow indicate clones, which were functionally analyzed in later experiments (see Figures 5–7).