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. 2019 Sep 20;10:2246. doi: 10.3389/fimmu.2019.02246

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Copyright © 2019 Rebl, Rebl, Verleih, Haupt, Köbis, Goldammer and Seyfert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of full-length factor Irak3 m and variants a, and m″ in HEK-293 cells. (A) Human HEK-293 cells were transfected with plasmids expressing the GFP-tagged Irak3 variants, the full-length factor m, truncated factor a, and splice variant m″. Cells were visualized 24 h after transfection with a confocal laser-scanning microscope. Nuclei were stained with Hoechst 33342 (blue). Scale bars represent 10 μm in all images. (B) Profile of fluorescence intensities (ordinate) recorded at specific locations (abscissa) across the cell following that path as indicated by the dotted arrows in 4A. (C) Live/dead assay of HEK-293 cells quantified the proportion of dead cells in the total cell count after the overexpression of full-length Irak3-m, truncated Irak3-a or spliced Irak3-m″ factors. The left part of each of the three dot plots shows the number of dead (PI-stained) GFP-negative cells and the right part shows the number of living GFP-positive (irak3-expressing) cells. One representative result is shown.