Figure 6.

Reporter-gene analysis assessing the influence of Irak-3 factors on NF-κB activity in HEK-293 cells. HEK-293 cells were co-transfected with constructs expressing the bovine TLR2 and the mammalian ELAM-driven NF-κB-luciferase reporter together with increasing amounts of the plasmids (gray-scaled columns) that express the irak3 variants m, a, m″, and l. (A) Fold-change values (ordinate) of the basal luciferase activity 24 h after transfection of Irak3-encoding plasmids, relative to the control (open columns) having received the empty plasmid only and no stimulation. The tertiary structures of the transfected irak3 variants are displayed below the diagram. (B,C) Fold-change values of the luciferase activity after stimulation of TLR2 signaling with 10 ng/ml Pam₂CSK₄ and 100 ng/ml FSL-1 for 24 h, (B) relative to unstimulated control cells having been transfected with the same amount of the respective irak3-encoding plasmids, (C) relative to stimulated control cells transfected with the empty plasmid. Mean values from 2 or 3 independent experiments, each assayed in triplicate, are given. Statistical significance compared with the control group was assessed using one-way ANOVA (^*p < 0.05; ^**p < 0.01; ^***p < 0.001).