FIGURE 1.

NGF-induced PC12 cell differentiation toward sympathetic neurons and expressions of GAP-43 and TH. (A) Immunofluorescent stains of GAP-43 (red, upper row) and TH (red, lower row). Nuclei were stained blue by DAPI. Note that NGF induced significant neurite outgrowth. Scale bar, 10 μm. (B,C) Real-time PCR and western blotting results of GAP-43 and TH expressions in PC12 cells treated or untreated with NGF. ^∗∗∗p < 0.001 vs. respective control. ^###p < 0.001 vs. respective control; n = 3 for each value.