Skip to main content
. 2019 Sep 20;13:415. doi: 10.3389/fncel.2019.00415

FIGURE 2.

FIGURE 2

Expressions of HCN channel isoforms in PC12 cells with or without NGF treatment. (A) Immunofluorescent stains of HCN1-4 proteins (green) in PC12 cells without NGF treatment. Scale bar, 10 μm for all subpanels. DAPI was used for cell nuclei staining (blue). (B) Representative real-time PCR amplification plots for HCN isoforms and GAPDH in PC12 cells without NGF treatment (cycle numbers vs. fluorescence). (C) Representative western blot electrophoresis bands in PC12 cells without NGF treatment. (D) Statistical results of real-time PCR and western blots of HCN isoforms in NGF-untreated PC12 cells. ∗∗∗p < 0.001 vs. the mRNA level of HCN1. ###p < 0.001 vs. the protein level of HCN1. (E) Representative western blots of HCN isoforms in NGF-treated and NGF-untreated PC12 cells. (F) Statistical results of protein levels for HCN channel isoforms in NGF-treated and NGF-untreated PC12 cells. (G) Statistical results of mRNA levels for HCN channel isoforms in NGF-treated and NGF-untreated PC12 cells.