FIGURE 3.

Editing does not significantly affect early stage neuronal differentiation. (Left) Representative images of 10-day-old cultures stained with antibodies to DCX (doublecortin, green) to visualize early stage neuronal differentiation, NeuN (red) to visualize more mature neurons and DAPI (blue) to assess total cell number. (Right) Quantification of positively stained cells for each marker indicate that there were no genotype-specific differences (ANOVA, Dunnett’s correction). Bars are the mean (SEM) of three biological replicates. Scale bar = 30 μm.