FIGURE 9.

The number of other endolysosomal vesicles is affected by editing. Rab5-positive objects in NCs is greater in 38–42-day-old Aβ42 samples. At a later culture age (63 days) both Aβ40 and Aβ42 samples have fewer LAMP1 objects. (Left) Maximum intensity projections of two adjacent optical sections (1 μm spacing) stained with anti-Rab5 antibody (early endosome marker, red) and DAPI (blue). (Right) The relative number of Rab5 puncta (normalized to DAPI) is greater in 38–42-day-old cultures for Aβ42 edited samples and less for both Aβ42 and Aβ40 edited samples in 63-day cultures (ANOVA, Dunnett’s corrected). Data are from individual NCs from three independent differentiations for 38–42-day-old cultures and two independent differentiations for 63-day cultures. Images for 63-day-old cultures can be found in Supplementary Figure S8. Mean values (±SEM) for 38–42-day-old samples are: H9 = 1 ± 0.1448, Aβ40 = 2.39 ± 0.2767, and Aβ42 = 4.80 ± 1.333; and for 63-day samples are: H9 = 1 ± 0.1584, Aβ40 = 0.586 ± 0.071, and Aβ42 = 0.4198 ± 0.0341. Scale bar = 10 μm