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. 2019 Sep 26;10(10):718. doi: 10.1038/s41419-019-1957-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunofluorescence staining for HA, Ki67, and CD44. Asterisks indicate ulcer margins. The numbers of Ki67+ cells or CD44+ cells were counted from five glands at ulcer margins. b Serial sections in situ hybridized for cycling crypt base columnar cell markers (Lgr5, Smoc2) and quiescent +4 stem cell markers (mTert, Lrig1, and Bmi1). Bar graph shows the numbers of positively stained cells of the indicated markers per crypt in each group. Arrows indicate Bmi1-positive cells in the crypt. n = 6 mice per group. *P < 0.05