Figure 6.

DHA is incorporated into RAW-ASC MΦ phospholipids at the expense of oleic acid (OA). RAW-ASC cells were incubated in serum-deprived media containing DHA-BSA complexes or BSA vehicle for 24 h. Cell phospholipids were extracted and analyzed for fatty acid content by GLC. In non-supplemented conditions, the major unsaturated fatty acid in the cell membrane is oleic acid (OA), which is the primary fatty acid component of fetal bovine serum. Incubation with DHA increased membrane content of DHA (A) while decreasing the membrane content of OA (B). See Table 1 for complete fatty acid profile. Data presented as mean ± SEM, n = 3. Asterisks indicate significant difference (**p < 0.01). Representative of two independent experiments.