Table 4.
Immunological analysis.
| ICU sepsis n = 18 | ICU controls n = 15 | Healthy controls n = 30 | Secondary infections n = 13 | No secondary infection n = 20 | p | SOFA <8 n = 14 | SOFA ≥ 8 n = 19 | p | |
|---|---|---|---|---|---|---|---|---|---|
| Lymphocytes (/mm3) | 992 [298–2,487] | 856 [298–2,246] | 2,042 [708–3,606] | 710 [319–1,517] | 1,056 [298–2,487] | 0.14 | 1,495 [298–2,487] | 658 [298–1,987] | 0.02 |
| CD4+ T Cells | 386 [124–1,510] | 405 [100–1,265] | 858 [366–1,731] | 320 [124–845] | 545 [100–1,510] | 0.13 | 729 [100–1,510] | 309 [124–1,265] | 0.02 |
| CD8+ T Cells | 183 [32–914] | 160 [23–687] | 448 [166–1,181] | 195 [23–687] | 165 [32–914] | 0.64 | 236 [23–914] | 129 [32–820] | 0.12 |
| CD19+ B Cells | 131 [20–380] | 163 [37–599] | 196 [72–713 | 150 [37–325] | 141 [20–599] | 0.54 | 173 [37–599] | 121 [20–410] | 0.31 |
| CD3−CD56+ NK Cells | 73 [18–320] | 68 [38–247] | 245 [57–787] | 71 [18–198] | 75 [31–320] | 0.28 | 103 [38–247] | 62 [18–320] | 0.03 |
| Monocytes HLA-DR expression | |||||||||
| MFI | 2649 [959–8,752] | 4882 [1,314–16,298] | 3,702 [959–12,941] | 3533 [1,048–16,298] | 0.52 | 6176 [1,314–16,298] | 3,479 [959–11,183] | 0.08 | |
| Ab/Cell | 5286 [2,089–16,832] | 7,882 [2,760–24,756] | 6,044 [2,089–24,756] | 6,959 [2,257–23,846] | 0.55 | 11,958 [2,760–24,756] | 6856 [2,089–15,804] | 0.05 | |
| Regulatory T Cells (CD4+CD25+FoxP3+) | |||||||||
| Absolute count (/mm3 of whole blood) | 9 [1–80] | 17 [5–64] | 11 [3–38] | 14 [1–80] | 0.67 | 17 [1–80] | 11 [3–34] | 0.04 | |
| ILC | |||||||||
| Total ILC (/mL of whole blood) | 1,293 [483–4,468] | 1,242 [326–4,085] | 1,632 [505–5,846] | 1,120 [326–4,085] | 1,277 [440–4,468] | 0.7 | 1,031 [326–4,468] | 1,264 [483–3,638] | 0.98 |
| ILC1 | |||||||||
| Absolute count (/mL) | 931 [282–3,478] | 512 [133–2,978] | 468 [178–1,980] | 514 [209–2,978] | 931 [133–3,478] | 0.67 | 505 [133–3,127] | 883 [282–3,478] | 0.57 |
| Percentage across ILCs | 66 [32–96] | 64 [27–83] | 30 [13–71] | 61 [27–77] | 69 [29–96] | 0.25 | 66 [27–82] | 68 [32–96] | 0.28 |
| ILC2 | |||||||||
| Absolute count (/mL) | 239 [29–1,592] | 147 [39–791] | 584 [117–1,597] | 294 [45–791] | 161 [29–1,592] | 0.3 | 211 [29–1,592] | 147 [29–518] | 0.60 |
| Percentage across ILCs | 20 [3–58] | 14 [3–43] | 28 [14–66] | 21 [7–39] | 13 [3–58] | 0.14 | 20 [6–44] | 14 [3–58] | 0.20 |
| ILC3 | |||||||||
| Absolute count (/mL) | 162 [41–469] | 258 [71–1,460] | 513 [83–2,424] | 261 [41–800] | 188 [41–1,460] | 0.74 | 180 [59–1,460] | 182 [41–644] | 0.41 |
| Percentage across ILCs | 13 [2–31] | 25 [8–49] | 32 [12–55] | 17 [5–49] | 14 [2–48] | 0.64 | 16 [3–49] | 14 [3–43] | 0.44 |
Variables are shown as median [min-max].