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. 2019 Sep 25;4(5):e00429-19. doi: 10.1128/mSphere.00429-19

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Copyright © 2019 Rainey et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Quantitative SWATH-MS proteomics revealed significant decrease in expression of the mutanobactin synthesizing complex in the smu_833 mutant. (A) Important features selected by a volcano plot with a fold change (FC) threshold (x) 1.5 and a t test threshold (y) of 0.05. The pink circles represent features above the threshold. (B) VIP plot. The graph displays the top 25 most important proteins identified by PLS-DA. These proteins contribute the most to the variance. Colored boxes on right indicate the relative concentrations of corresponding proteins for samples from the wild-type and smu_833 mutant strains. VIP is a weighted sum of squares of the PLS-DA loadings, taking into account the amount of explained Y-variable in each dimension.