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. 2019 Aug 10;13(10):2142–2159. doi: 10.1002/1878-0261.12552

Figure 2.

Figure 2

Progranulin interacts with and positively regulates STAT3 activation in CRC cells. (A) Total proteins extracted from HCT‐116 and HT‐29 cells were immunoprecipitated by an anti‐human STAT3 antibody and then subjected to immunoblotting analysis using progranulin, STAT3, and β‐actin antibodies. One of three representative experiments in which similar results were obtained is shown. (B) Progranulin ASO downregulates progranulin and p‐STAT3 Tyr705 protein expression in CRC cells. HCT‐116 and HT‐29 cells were transfected with either scrambled (Scr) antisense oligonucleotide (ASO) (used at 400 nm) or increasing doses of progranulin ASO as indicated. Progranulin, p‐STAT3 Tyr705, and β‐actin were analyzed by immunoblotting. One of three representative experiments is shown together with the densitometry analysis in the lower insets. Values are expressed in arbitrary units and are the mean ± SEM. Differences were compared using the two‐tailed Student's t‐test (Scr ASO‐transfected cells vs progranulin ASO‐transfected cells, *P < 0.05, **P < 0.01, ***P < 0.001).