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. 2019 Aug 21;13(10):2178–2193. doi: 10.1002/1878-0261.12559

Figure 2.

Figure 2

Induction of apoptosis by CDKI‐73 in HCT 116 and HT29 cells. (A) Cell cycle analysis of HCT 116 and HT29 cells after exposure to 0.25 μm CDKI‐73 for 24 h. (B) Apoptosis analysis of HCT 116 and HT29 cells after incubation with CDKI‐73 for 48 h by annexin V/PI assay. Co‐treatment of CDKI‐73 with Z‐VAD‐FMK (20 μm) was performed to demonstrate the caspase independency. Representative cytometric plots are presented. (C) Quantification of apoptotic cells. Untreated cells were used as negative control. Doxorubicin (Dox, 1 μm) was used as positive control. Data presented as mean ± SEM of three independent experiments. For each treatment condition, 10 000 events were recorded and cellular debris was excluded from the analysis. *P < 0.05 compared with negative control; # P < 0.05 compared with respective treatment without Z‐VAD‐FMK determined by ANOVA followed by Student–Newman–Keuls test.