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. 2019 Aug 20;13(10):2107–2120. doi: 10.1002/1878-0261.12549

Figure 3.

Figure 3

(A) Immunoprecipitation with a CD300f polyclonal antibody reveals multiple forms of CD300f in AML cell lines and primary AML cell samples. CD300f was immunoprecipitated from biotinylated membrane lysates; HEL, HL‐60, U937, THP‐1, and a primary AML9 membrane lysate using goat anti‐LMIR3 or goat serum. Immunoprecipitated protein was detected with streptavidin–HRP and ECL substrate. (B) CD300f exon 4 splice variants or (C) CD300fC was compared between healthy CB‐derived CD34+ HSPC and primary AML samples (flow‐sorted or blast count > 90%). Statistical analysis was performed using t‐tests. *P < 0.05.